Material

Plasmids

pRC/CMV-PAGFP-hTau352wt (eukaryotic expression vector coding for human tau (352 amino acids) with N-terminal PAGFP-tag);
(Gauthier-Kemper et al., 2011),
<Download as GenBank File>
pRC/CMV-PAGFP-hTau441wt (eukaryotic expression vector coding for human tau (441 amino acids) with N-terminal PAGFP-tag);
(Gauthier-Kemper et al., 2011),
<Download as GenBank File>
pRC/CMV-PAGFP-hG3BP1 (eukaryotic expression vector coding for human ras-GAP Src homology 3 domain-binding protein 1 (G3BP1) with N-terminal PAGFP-tag);
(Moschner et al., 2014),
<Download as GenBank File>
pSems-GAP43wt-HaloTag (eukaryotic expression vector coding for growth associated protein 43 (GAP43) with C-terminal HaloTag);
(Gauthier-Kemper et al., 2014),
<Download as GenBank File>
pSems-HaloTag-hTau441wt (eukaryotic expression vector coding for human tau (441 amino acids) with N-terminal HaloTag);
(Janning et al., 2014),
<Download as GenBank File>

Application Note: Photoactivatable green fluorescent protein (PA-GFP):a valuable tool for selective photolabelling of proteins and cells (together with Nikon Corporation) (Application note)
Software suite "SegMorphOS" (see: Sündermann et al. (2014) Shearlet-analysis of confocal laser-scanning microscopy images to extract morphological features of neurons. Neuromethods 87:293-303): please mail for a download link
Python Sholl Analysis Scripts (see: Golovyashkina et al. (2014) Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins. Neuromethods 87:251-262) (Python-Script for Sholl Analysis);(Config-File for Sholl Analysis)

Scripts for data analysis are available at: (Department-of-Neurobiology at GitHub)