Material

Plasmids

• pRC/CMV-PAGFP-hTau352wt (eukaryotic expression vector coding for human tau (352 amino acids) with N-terminal PAGFP-tag);
    (Gauthier-Kemper et al., 2011),
    <Download as GenBank File>
  
• pRC/CMV-PAGFP-hTau441wt (eukaryotic expression vector coding for human tau (441 amino acids) with N-terminal PAGFP-tag);
    (Gauthier-Kemper et al., 2011),
    <Download as GenBank File>
  
• pRC/CMV-PAGFP-hG3BP1 (eukaryotic expression vector coding for human ras-GAP Src homology 3 domain-binding protein 1 (G3BP1) with N-terminal PAGFP-tag);
    (Moschner et al., 2014),
    <Download as GenBank File>
  
• pSems-GAP43wt-HaloTag (eukaryotic expression vector coding for growth associated protein 43 (GAP43) with C-terminal HaloTag);
    (Gauthier-Kemper et al., 2014),
    <Download as GenBank File>
  
• pSems-HaloTag-hTau441wt (eukaryotic expression vector coding for human tau (441 amino acids) with N-terminal HaloTag);
    (Janning et al., 2014),
    <Download as GenBank File>
  

Antibodies

• monoclonal antibody against gravin (JP74)
• monoclonal antibody against GAP43 (clone JP15)
• monoclonal antibody against O-glycosylated_neurofilament M (NF-M) (clone NL6)
• monoclonal antibody against nestin (clone JP63)

Application Notes and Scripts

• Application Note: Photoactivatable green fluorescent protein (PA-GFP):a valuable tool for selective photolabelling of proteins and cells (together with Nikon Corporation) (Application note)
• Software suite "SegMorphOS" (see: Sündermann et al. (2014) Shearlet-analysis of confocal laser-scanning microscopy images to extract morphological features of neurons. Neuromethods 87:293-303): please mail for a download link
• Python Sholl Analysis Scripts (see: Golovyashkina et al. (2014) Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins. Neuromethods 87:251-262) (Python-Script for Sholl Analysis);(Config-File for Sholl Analysis)